Fermentation with cyclic pulse-pause feeding

本发明涉及生产有价值的化合物的方法，包括如下步骤：a)在发酵培养基中发酵丝状细菌或真菌(例如链霉属菌株或曲霉属菌株)，在发酵期间采用循环脉冲定量给料/暂停的方式在所述发酵培养基中加入糖类，其中所述脉冲定量给料的时间短于暂停时间；和b)从发酵培养液中回收有价值的化合物。 A process for the production of a valuable compound, comprising the steps of a) fermentation of a filamentous bacterial or fungal strain (e.g. a Streptomyces strain or an Aspergillus strain) in a fermentation medium wherein a carbohydrate during fermentation is added in a cyclic pulse dosing/pause way, wherein the pulse dosing time is shorter than the pause time and b) recovery of the valuable compound from the fermentation broth.