Reptilase and its production process and application

The reptilase is extracted from snake poison of pallas pit viper, and its SDS-polyacrylamide gel electrophoresis exhibits three bands of molecular weight 54000 D, 34000 D and 15000 D separately. The reptilase producing process of the present invention includes the following steps: taking snake poiso...

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Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The reptilase is extracted from snake poison of pallas pit viper, and its SDS-polyacrylamide gel electrophoresis exhibits three bands of molecular weight 54000 D, 34000 D and 15000 D separately. The reptilase producing process of the present invention includes the following steps: taking snake poison of pallas pit viper, dissolving in buffering liquid, centrifugal deposition, elution in saturated phenol solution to obtain the grade-I reptilase product; dissolving the grade-I reptilase product in buffering liquid, ion exchange column chromatography and elution with buffering liquid to obtain refined reptilase liquid. The reptilase medicine of the present invention has very low toxicity, safe clinical application and wide application foreground. 本发明公开了一种蛇毒凝血酶及其生产方法与应用。本发明所提供的蛇毒凝血酶,自白眉蝮蛇蛇毒中提取;该凝血酶的SDS-聚丙稀酰胺凝胶电泳呈三条带,三条带的分子量分别为:54000D,34000D及15000D。本发明生产蛇毒凝血酶的方法,包括如下步骤:(1)取白眉蝮蛇蛇毒,用缓冲液溶解,离心沉淀,酚饱和溶液洗脱,得到蛇毒凝血酶I级酶制品;(2)将所得的蛇毒凝血酶I级酶制品用缓冲液溶解,再经离子交换柱层析,用缓冲液洗脱,得精制蛇毒酶液。本发明的凝血药物毒性极弱,临床使用安全,具有广泛的应用前景。