Immune colloidal gold reagent for detecting chloromycetin and preparation method thereof

The invention is a kind of immune colloidal gold reagent for measuring the oxymycin and the manufacturing method. The reagent includes a sample cushion, a colloidal gold combination cushion, aeotic acid cellulose film, a suction cushion and a PVC back lining, one end of the PVC back lining is adhered with sample cushion, colloidal gold combination cushion in order, the middle part is adhered with aeotic acid cellulose film, the other end is adhered with a suction cushion. The character lies in: the colloidal gold combination cushion is enclosed with a kind of oxymycin resisting specificity single clone antibody-colloidal gold label, the aeotic acid cellulose film is enclosed by oxymycin-BSA couplings and sheep (rabbit) antiplague IgG. The reagent has a strong specificity, high sensitivity, quick and simple, cheap, and convenient. 检测氧霉素的免疫胶体金试剂及制备方法，该试剂包括样品垫、结合垫、硝酸纤维素膜、吸水垫和PVC背衬，PVC背衬一端依次粘附样品垫、结合垫，中间粘附硝酸纤维素膜，另一端粘附吸水垫，其特征在于结合垫上包被了抗氯霉素特异性单克隆抗体-胶体金标记物，硝酸纤维素膜上包被了氯霉素-BSA偶联物和羊(兔)抗鼠IgG。该试剂是一种特异性强、敏感性高、简易快速、费用低廉、能现场检测，全过程只需30分钟，操作人员无需专业培训，按说明书即可完成操作。