Complex combinatorial chemical libraries encoded with tags

本文提供了编码组合化学，其中用有机分子记录连续合成方法，这些有机分子作为相同或不同的信息量子确定反应物和阶段的选择。各种产物可在多步合成方法中产生，如低聚物和合成的非重复性有机分子。有多族化合物可用作识别剂，其中取代基的数量和/或位置可确定该选择。另外，可使用可测定的官能团，如放射性同位素、荧光、卤素等，其中两个不同基团的存在和比例可用于确定阶段或选择。特别是，可用多种识别剂以提供二进制或高级代码，以便仅用少数可分离标示物就可确定多种选择。可筛选颗粒的所需特性，特别是结合亲合力，其中产物可与颗粒分离或留在颗粒上。可通过释放标示物和分析确定颗粒的反应历程来测量定具有所述特性的颗粒的反应历程。 Encoded combinatorial chemistry is provided, where sequential synthetic schemes are recorded using organic molecules, which define choice of reactant, and stage, as the same or different bit of information. Various products can be produced in the multi-stage synthesis, such as oligomers and synthetic non-repetitive organic molecules. Conveniently, nested families of compounds can be employed as identifiers, where number and/or position of a substituent define the choice. Alternatively, detectable functionalities may be employed, such as radioisotopes, fluorescers, halogens, and the like, where presence and ratios of two different groups can be used to define stage or choice. Particularly, pluralities of identifiers may be used to provide a binary or higher code, so as to define a plurality of choices with only a few detachable tags. The particles may be screened for a characteristic of interest, particularly binding affinity, where the products may be detached from the particle or retained on the particle. The reaction history of the particles which are positive for the characteristic can be determined by the release of the tags and analysis to define the reaction history of the particle.