New-type recombinant tissue factor inhibiting peptide and its prepn and application

The present invention belongs to the field of biological technology, and is especially a new kind of recombinant tissue factor inhibiting peptide with anticoagulant and thrombosis resisting activity and its preparation. Based on the structure and biochemical characteristic analysis of whole length t...

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Bibliographische Detailangaben
Hauptverfasser:	HAOYAN SONG, MIN YU
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention belongs to the field of biological technology, and is especially a new kind of recombinant tissue factor inhibiting peptide with anticoagulant and thrombosis resisting activity and its preparation. Based on the structure and biochemical characteristic analysis of whole length tissue factor, the present invention designs the structure of novel recombinant tissue factor inhibiting peptide, recombines mutant tissue factor inhibiting peptide gene and prokaryotic expression vector pLK-4, transforms proteinase defective colibacillus and screens high expression engineering bacteria. Through engineering bacteria fermentation, high pressure breaking of bacteria, dissolving inclusion body, renaturation and purification, recombinant tissue factor inhibiting peptide is obtained.