Method for preparing recombinant human plasminogen kringle 5 mutant protein rhpk-5

Method for preparing recombinant human plasminogen kringle 5 mutant protein rhpk-5

A process for preparing recombinant human plasminogen kringle 5 (rhPK-5) and its mutant includes designing the gene of rhPK-5 by genetic engineering and protein engineering, constructing prokaryotic expression vector pBV220/rhPK-5, and respectively transforming 4 kinds of colibacillus to obtain thei...

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Bibliographische Detailangaben
Hauptverfasser: YI YANG, BO NIU, XIANJIU CHEN
Format: Patent
Sprache:eng
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BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
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PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
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Zusammenfassung:A process for preparing recombinant human plasminogen kringle 5 (rhPK-5) and its mutant includes designing the gene of rhPK-5 by genetic engineering and protein engineering, constructing prokaryotic expression vector pBV220/rhPK-5, and respectively transforming 4 kinds of colibacillus to obtain their expressions. The resultant rhPK-5 has the bioactivity to suppress the neogenesis of blood vessel.