Macroporous chitosan beads and preparation method thereof

The present invention relates to macroporous chitosan beads having 5-200 mum in size of relatively large and uniform pores that are distributed from surface to core region, and a preparation method thereof compring the following steps; by dropping chitosan solution, aqueous chitosan solution or their mixture into the low-temperature of organic solvent or liquid nitrogen; and by regulating pore size by phase separation method via temperature difference. The macroporous chitosan beads of the present invention make cell culture more efficient than the previous substrate, since cell can attach to them efficiently due to their large surface area, it is easy for cell to be injected into them and cell attached to them can exist longer due to their three-dimensional structure, therefore they can be used for a study about production of protein, abtibiotics, anticancer agent, polysaccharide, physiologically active agent, animal hormone, or plant hormone as well as a study about substitution of metabolic organs, cartilage or bone.