Method of in vitro culturing medicine ox gallstone with natural ox gall

Natural ox gall is first taurine regulated and bacteria fermented to prepare fermented ox gall. The fermented ox gall is gall is then mixed with clear saturated calcium salt solution through stirring, heated to boil, filtered to obtain red brown precipitate, which is stirred, washed, filtered to eliminate supernatant and mixed with bilirubin, cholalic acid, deoxycholic acid, inorganic salt and other effective components to obtain composite bilirubin calcium. Fermented ox gall and bilirubin calcium in certain proportion are mixed and the pH of mixture is regulated to below 6.8 and directional off axis rotated to form gallstone, and the gallstone is rest cultured, taken out and dried. The said process can obtain ox gallstone product with the same characteristics, structure, component and medicinal effect as high quality natural bezoar.