Process for preparing fusion protein used for glucose sensor

A process for preparing the fusion protein used for glucose sensor includes such step as synthesizing DNA chain for coding polylysine, annealing it at 65 deg.C to become dual-chain short DNA fragment, gene splicing to create the expression carrier pPICGLT of fusion protein, linearizing it by restric...

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Hauptverfasser:	WEIHONG XIE, LIQUN CHEN, XIANEN ZHANG
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Zusammenfassung:	A process for preparing the fusion protein used for glucose sensor includes such step as synthesizing DNA chain for coding polylysine, annealing it at 65 deg.C to become dual-chain short DNA fragment, gene splicing to create the expression carrier pPICGLT of fusion protein, linearizing it by restriction endonuclease, transferring it into yeast, screening yeast recon, extracting genom DNA of yeastfor test, expressing and purifying fusion protein, culturing transformant in MM culture medium at 30 deg.C for 72 hr while adding methanol once per 24 hr, linking fusion protein with ceptor, preparing glucose sensor and electrochemical test. Resultant sensor features wide linear range up to 45 mmol/L, great amplitude of response signal and long storage period.