Piglet red dysentery welch's fusiform bacillus alpha-beta toxin bivalent gene engineering vaccine and preparing method
The present invention relates to young pig red dysentery clostridium welchii alpha-beta toxin bivalent genetic engineering vaccine and its preparation method. It is characterized polymerase chain reaction is used to amplify alpha and beta tovic protective antigen gene from clostridium welchii chromo...
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| creator | GUANGSEN WEI |
| description | The present invention relates to young pig red dysentery clostridium welchii alpha-beta toxin bivalent genetic engineering vaccine and its preparation method. It is characterized polymerase chain reaction is used to amplify alpha and beta tovic protective antigen gene from clostridium welchii chromosome, then passes through separation, inactivation, endo enzyme enzymolysis to obtain alpha toxic gene and beta toxic gene, and construct genetic engineering bacterial strain BL21(DE3)(pxCPAB2) containing alpha-beta toxic fusion gene expressing carrier, the effective antigen component of said bacterial strain exists in the inside of thallus, it can gradually release and act continuously. |
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It is characterized polymerase chain reaction is used to amplify alpha and beta tovic protective antigen gene from clostridium welchii chromosome, then passes through separation, inactivation, endo enzyme enzymolysis to obtain alpha toxic gene and beta toxic gene, and construct genetic engineering bacterial strain BL21(DE3)(pxCPAB2) containing alpha-beta toxic fusion gene expressing carrier, the effective antigen component of said bacterial strain exists in the inside of thallus, it can gradually release and act continuously.</description><edition>7</edition><language>eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; HUMAN NECESSITIES ; HYGIENE ; MEDICAL OR VETERINARY SCIENCE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2001</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20010905&DB=EPODOC&CC=CN&NR=1311037A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25543,76293</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20010905&DB=EPODOC&CC=CN&NR=1311037A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>GUANGSEN WEI</creatorcontrib><title>Piglet red dysentery welch's fusiform bacillus alpha-beta toxin bivalent gene engineering vaccine and preparing method</title><description>The present invention relates to young pig red dysentery clostridium welchii alpha-beta toxin bivalent genetic engineering vaccine and its preparation method. It is characterized polymerase chain reaction is used to amplify alpha and beta tovic protective antigen gene from clostridium welchii chromosome, then passes through separation, inactivation, endo enzyme enzymolysis to obtain alpha toxic gene and beta toxic gene, and construct genetic engineering bacterial strain BL21(DE3)(pxCPAB2) containing alpha-beta toxic fusion gene expressing carrier, the effective antigen component of said bacterial strain exists in the inside of thallus, it can gradually release and act continuously.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>HUMAN NECESSITIES</subject><subject>HYGIENE</subject><subject>MEDICAL OR VETERINARY SCIENCE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2001</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqFjLEKwjAYhLs4iPoM_ptTwdLBWYriJA7u5W9yTQNpEpI02re3iLvTcR_33brID60MEgVIknOETQgzvWDEcIjUT1H3LozUsdDGTJHY-IHLDokpube21OnMZtFIwYJglbZA0FZRZiGWQmwl-QDPXzoiDU5ui1XPJmL3y02xv16eza2Edy2iZ7G8pba5V3VVHevTuf6_-AA4gET8</recordid><startdate>20010905</startdate><enddate>20010905</enddate><creator>GUANGSEN WEI</creator><scope>EVB</scope></search><sort><creationdate>20010905</creationdate><title>Piglet red dysentery welch's fusiform bacillus alpha-beta toxin bivalent gene engineering vaccine and preparing method</title><author>GUANGSEN WEI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN1311037A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng</language><creationdate>2001</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>HUMAN NECESSITIES</topic><topic>HYGIENE</topic><topic>MEDICAL OR VETERINARY SCIENCE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>GUANGSEN WEI</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>GUANGSEN WEI</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Piglet red dysentery welch's fusiform bacillus alpha-beta toxin bivalent gene engineering vaccine and preparing method</title><date>2001-09-05</date><risdate>2001</risdate><abstract>The present invention relates to young pig red dysentery clostridium welchii alpha-beta toxin bivalent genetic engineering vaccine and its preparation method. It is characterized polymerase chain reaction is used to amplify alpha and beta tovic protective antigen gene from clostridium welchii chromosome, then passes through separation, inactivation, endo enzyme enzymolysis to obtain alpha toxic gene and beta toxic gene, and construct genetic engineering bacterial strain BL21(DE3)(pxCPAB2) containing alpha-beta toxic fusion gene expressing carrier, the effective antigen component of said bacterial strain exists in the inside of thallus, it can gradually release and act continuously.</abstract><edition>7</edition><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Piglet red dysentery welch's fusiform bacillus alpha-beta toxin bivalent gene engineering vaccine and preparing method |
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