Piglet red dysentery welch's fusiform bacillus alpha-beta toxin bivalent gene engineering vaccine and preparing method

The present invention relates to young pig red dysentery clostridium welchii alpha-beta toxin bivalent genetic engineering vaccine and its preparation method. It is characterized polymerase chain reaction is used to amplify alpha and beta tovic protective antigen gene from clostridium welchii chromo...

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1. Verfasser:	GUANGSEN WEI
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to young pig red dysentery clostridium welchii alpha-beta toxin bivalent genetic engineering vaccine and its preparation method. It is characterized polymerase chain reaction is used to amplify alpha and beta tovic protective antigen gene from clostridium welchii chromosome, then passes through separation, inactivation, endo enzyme enzymolysis to obtain alpha toxic gene and beta toxic gene, and construct genetic engineering bacterial strain BL21(DE3)(pxCPAB2) containing alpha-beta toxic fusion gene expressing carrier, the effective antigen component of said bacterial strain exists in the inside of thallus, it can gradually release and act continuously.