Method for measuring 5'-nucleotidase activity and diagnostic reagent kit of 5'-nucleotidase

The present invention relates to 5í»-nucleotidase activity detecting method and 5í»-nucleotidase diagnosing reagent kit in medical detecting technology. The 5í»-nucleotidase diagnosing reagent kit includes buffering liquid, adenosine monophosphate, 2-ketoglutarate, reductive coenzyme, adenosine deam...

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Bibliographische Detailangaben
1. Verfasser:	ERZHONG WANG
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to 5í»-nucleotidase activity detecting method and 5í»-nucleotidase diagnosing reagent kit in medical detecting technology. The 5í»-nucleotidase diagnosing reagent kit includes buffering liquid, adenosine monophosphate, 2-ketoglutarate, reductive coenzyme, adenosine deaminase, glutamate dehydrogenase, stabilizer, etc. Through mixing the sample and reagent in certain volume ratio to produce enzyme coupling reaction, and detecting in biochemical analyzer the main wavelength absorbency change speed, the activity of 5í»-nucleotidase is calculated. The present invention can obtain the measurement result in biochemical analyzer in high sensitivity, high precision and no contamination of various foreign and internal matters.