Circuit arrangement for injecting nucleic acids and other biologically active molecules

Apparatus for electrophoretic transfer of nucleic acids, peptides, proteins and/or other biologically active molecules into eukaryotic cells comprises two capacitors, each connected to a high voltage source. Apparatus for electrophoretic transfer of nucleic acids, peptides, proteins and/or other bio...

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Bibliographische Detailangaben
1. Verfasser:	H.M. HARTMANN,G. RIEMEN,K. ROTHMANN,C. TILL,L. OTTEROG,M. WEIGL,R. CHRISTIE,E. ROBACH,J. HELFRICH,H. WESENDOFF,G. HIBENKETEN
Format:	Patent
Sprache:	eng
Schlagworte:	APPARATUS FOR CONVERSION BETWEEN AC AND AC, BETWEEN AC AND DC,OR BETWEEN DC AND DC, AND FOR USE WITH MAINS OR SIMILAR POWERSUPPLY SYSTEMS APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BASIC ELECTRONIC CIRCUITRY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CONTROL OR REGULATION THEREOF CONVERSION OF DC OR AC INPUT POWER INTO SURGE OUTPUTPOWER CONVERSION OR DISTRIBUTION OF ELECTRIC POWER CULTURE MEDIA ELECTRICITY ELECTROTHERAPY ENZYMOLOGY GENERATION HUMAN NECESSITIES HYGIENE MAGNETOTHERAPY MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS PULSE TECHNIQUE RADIATION THERAPY SPIRITS ULTRASOUND THERAPY VINEGAR WINE
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Beschreibung
Zusammenfassung:	Apparatus for electrophoretic transfer of nucleic acids, peptides, proteins and/or other biologically active molecules into eukaryotic cells comprises two capacitors, each connected to a high voltage source. Apparatus for electrophoretic transfer of nucleic acids, peptides, proteins and/or other biologically active molecules into eukaryotic cells comprises two capacitors (7, 8), each connected to a high voltage source (5, 6). Each capacitor discharges via a power transistor (9, 10) into a cuvette holding the cell sample. The first capacitor is charged with a preset voltage U 1 and the second with a voltage U 2 given by the equation: U 2 = R x I 2 x K 2, R 1the resistance of the cuvette and suspension in it I 2the desired current K 2a correction factor based on the properties of thee cuvette ACTIVITY : None given. MECHANISM OF ACTION : Gene therapy. No supporting data provided.