Process for producing recombinant insulin from novel fused protein

This invention relates to a DNA encoding a fusion protein of formula (I) ÄYÜ-ÄX1Ü-ÄB-chainÜ-ÄX2Ü-ÄLinkerÜ-ÄX3Ü-ÄA-chainÜ wherein Y is a leader peptide sequence for the expression and secretion of the protein, comprising at least one amino acid residue; X1 is an amino acid sequence which is cleavable...

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Bibliographische Detailangaben
Hauptverfasser:	SATO SEIJI, HIGASHIKUNI NAOHIKO, OKA SHUSAKU
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	This invention relates to a DNA encoding a fusion protein of formula (I) ÄYÜ-ÄX1Ü-ÄB-chainÜ-ÄX2Ü-ÄLinkerÜ-ÄX3Ü-ÄA-chainÜ wherein Y is a leader peptide sequence for the expression and secretion of the protein, comprising at least one amino acid residue; X1 is an amino acid sequence which is cleavable enzymatically or chemically; B-chain is the amino acid sequence of insulin B chain; X2 is an amino acid sequence which is cleavable enzymatically; Linker is a linker sequence comprising at least one amino acid residue; X3 is an amino acid sequence which is cleavable enzymatically; and A- chain is the amino acid sequence of insulin A chain, and to a method for producing insulin using an expression system containing the DNA in high efficiency and high yield.