Method for producing recombinant human interleukin-6

The production method of recombinant human interleukin-6 (rhIL-6) includes: artificial synthesis of up-stream and downstream oligonucleotide primers; using human IL-6DNA gene as template and using the described primers to make PCR amplification; using EcoRI and BamHI to respectively enzyme-cut pBV220 plasmid and PCR amplified hIL-6 gene fragment, then using T4DNA ligase to make connection; using the formed expression plasmid to convert colibacillus, inducing and culturing the formed transformant and recovering rhIL-6 from culture and purifying it. The protein expressed by transformant in the invented method can be 45% of total protein of thallus, and the purity of rhIL-6 can be above 95%.