Microfluidic device for rapidly detecting bacteria through gradient diffusion as well as preparation method and application of microfluidic device

The invention provides a micro-fluidic device for rapidly detecting bacteria through gradient diffusion as well as a preparation method and application of the micro-fluidic device. Gradient diffusion, rapid enrichment and detection of pseudomonas aeruginosa are realized through a core component beta...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	YAN KUN, WANG DONG, YANG CHENGUANG, LU ZHENTAN, WANG WENWEN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS SPIRITS THEIR RELEVANT APPARATUS TRANSPORTING VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The invention provides a micro-fluidic device for rapidly detecting bacteria through gradient diffusion as well as a preparation method and application of the micro-fluidic device. Gradient diffusion, rapid enrichment and detection of pseudomonas aeruginosa are realized through a core component beta-D galactose monomer/chitosan composite film in the microfluidic device. The beta-D galactose monomer/chitosan composite film has strong adhesion and can be used for quickly enriching bacteria and enhancing the detection capability of the bacteria, and the beta-D galactose monomer has specific adsorption capability on pseudomonas aeruginosa, so that the specific detection of the bacteria can be realized; the chitosan and the grafted beta-D galactose monomer can cooperate with each other, so that the bacteria are rapidly enriched due to strong adhesion, the bacteria form a gradient diffusion effect on the membrane, and the gradient diffusion rapid detection function of the bacteria is further realized. 本发明提供了一种梯度扩散快