Oligonucleotide composition, kit and method for detecting allopurinol medication anaphylaxis and application

The invention relates to the technical field of allopurinol medication anaphylaxis, in particular to an oligonucleotide composition, a kit, a method and application for detecting allopurinol medication anaphylaxis. The composition comprises a molecular beacon 1, a molecular beacon 2 and a sequence 6. The kit comprises the composition, ABTS and hydrogen peroxide. The allele of HLA-B * 5801 is amplified through the oligonucleotide composition for detection, so that the serious skin adverse reaction caused by allopurinol medication is detected or prevented. The method has the characteristics of simplicity in operation, high sensitivity, specificity and accuracy and low cost. 本发明涉及别嘌醇用药过敏反应的技术领域，具体涉及检测别嘌醇用药过敏反应的寡核苷酸组合物、试剂盒、方法及用途。该组合物包括分子信标1、分子信标2、序列6。该试剂盒包括该组合物、ABTS及双氧水。本发明通过寡核苷酸组合物扩增HLA-B*5801的等位基因进行检测，从而检测或预防别嘌醇用药的严重皮肤不良反应的发生。本发明具有操作简单，灵敏度、特异性和准确率高，成本低的特点。