FRET-based fluorescent probe, preparation method and application in detection of sialic acid

The invention provides a fluorescent probe based on FRET (Fluorescence Resonance Energy Transfer). The fluorescent probe based on the FRET comprises a structure shown in a formula 1: # imgabs0 # R which is selected from one of H, a sulfonic group and sulfonate. The invention also provides a preparation method of the fluorescent probe and application of the fluorescent probe in detection of sialic acid. The fluorescent probe is simple in preparation method, has good single-excitation dual-emission performance, is used as a probe to realize fluorescence ratio detection of sialic acid, and can avoid the problem that single-wavelength fluorescence detection is easily influenced by probe concentration, environmental factors and the like. And the detection limit reaches 1.83 [mu] M. 本发明提供一种基于FRET的荧光探针，所述基于FRET的荧光探针包括式1所示结构：#imgabs0#R选自H、磺酸基及磺酸盐中的一种。本发明还提供该荧光探针的制备方法以及该荧光探针在检测唾液酸中的应用。该荧光探针制备方法简单，有良好的单激发双发射性能，用作探针实现荧光比率检测唾液酸，可以避免单波长荧光检测易受探针浓度、环境因素等影响的问题。检测限达到1.83μM。