Method for preparing adjuvant

The present invention provides a method for producing a homogeneous and heterogeneous adjuvant formulation comprising a liposome bilayer comprising (a) monophosphoryl lipid A (MPLA), (b) a saponin, and (c) a liposome composition comprising (i) at least one phospholipid selected from the group consisting of phosphatidylcholine (PC) and/or phosphatidylglycerol (PG), wherein the phospholipid is selected from the group consisting of dimyristoyl phosphatidylcholine (DMPC), dipalmitoyl phosphatidylcholine (DPPC), distearoyl phosphatidylcholine (DSPC), dimyristoyl phosphatidylglycerol (DMPG), dipalmitoyl phosphatidylglycerol (DPPG), distearoyl phosphatidylglycerol (DSPG), and combinations thereof, and (ii) cholesterol, wherein the molar percent concentration of cholesterol in the liposome composition is greater than 50% (mol/mol), and the invention further provides adjuvant formulations produced by the method. 本发明提供用于生产包含脂质体双层的均质和异质佐剂制剂的方法，该脂质体双层包含(a)单磷酰脂质A(MPLA)、(b)皂苷及(c)脂质体组合物，所述脂质体组合物包含(i)至少一种选自磷脂酰胆碱(PC)和/或磷脂酰甘油(