Method for detecting drug resistance of mycobacterium tuberculosis based on nanopore targeted sequencing technology
The invention discloses a method for detecting drug resistance of mycobacterium tuberculosis based on a nanopore targeted sequencing technology, and relates to the technical field of microbiological identification. Mycobacterium tuberculosis genome DNA in a sample to be detected is used as a templat...
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| creator | BAO XUNDI KAN XIAOHONG ZHANG JINGJING ZHENG YABIAO |
| description | The invention discloses a method for detecting drug resistance of mycobacterium tuberculosis based on a nanopore targeted sequencing technology, and relates to the technical field of microbiological identification. Mycobacterium tuberculosis genome DNA in a sample to be detected is used as a template, and a primer group designed aiming at a drug-resistant gene is used for PCR amplification; the obtained PCR amplification product is subjected to nanopore sequencing platform library building and sequencing, and whether the mycobacterium tuberculosis is resistant to drugs or not is predicted according to sequencing data; according to the invention, a nanopore sequencing platform and a multiplex PCR amplification technology are utilized to realize rapid detection of mycobacterium tuberculosis related drug-resistant genes and mutations, and the detection accuracy and efficiency are improved.
本发明公开了一种基于纳米孔靶向测序技术检测结核分枝杆菌耐药性的方法，涉及微生物鉴定技术领域，本发明以待测样品中的结核分枝杆菌基因组DNA为模板，利用针对耐药基因设计的引物组进行PCR扩增，将得到的PCR扩增产物进行纳米孔测序平台建库和测序，根据测序 |
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本发明公开了一种基于纳米孔靶向测序技术检测结核分枝杆菌耐药性的方法，涉及微生物鉴定技术领域，本发明以待测样品中的结核分枝杆菌基因组DNA为模板，利用针对耐药基因设计的引物组进行PCR扩增，将得到的PCR扩增产物进行纳米孔测序平台建库和测序，根据测序</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2024</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20241105&DB=EPODOC&CC=CN&NR=118895373A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76516</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20241105&DB=EPODOC&CC=CN&NR=118895373A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>BAO XUNDI</creatorcontrib><creatorcontrib>KAN XIAOHONG</creatorcontrib><creatorcontrib>ZHANG JINGJING</creatorcontrib><creatorcontrib>ZHENG YABIAO</creatorcontrib><title>Method for detecting drug resistance of mycobacterium tuberculosis based on nanopore targeted sequencing technology</title><description>The invention discloses a method for detecting drug resistance of mycobacterium tuberculosis based on a nanopore targeted sequencing technology, and relates to the technical field of microbiological identification. Mycobacterium tuberculosis genome DNA in a sample to be detected is used as a template, and a primer group designed aiming at a drug-resistant gene is used for PCR amplification; the obtained PCR amplification product is subjected to nanopore sequencing platform library building and sequencing, and whether the mycobacterium tuberculosis is resistant to drugs or not is predicted according to sequencing data; according to the invention, a nanopore sequencing platform and a multiplex PCR amplification technology are utilized to realize rapid detection of mycobacterium tuberculosis related drug-resistant genes and mutations, and the detection accuracy and efficiency are improved.
本发明公开了一种基于纳米孔靶向测序技术检测结核分枝杆菌耐药性的方法，涉及微生物鉴定技术领域，本发明以待测样品中的结核分枝杆菌基因组DNA为模板，利用针对耐药基因设计的引物组进行PCR扩增，将得到的PCR扩增产物进行纳米孔测序平台建库和测序，根据测序</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>COMPOSITIONS THEREOF</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2024</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNzDEOgkAQRmEaC6PeYTyABSFGLA3R2GhlT5bdn4UEZnB3tuD2YuIBrF7z8q2z-IB24qiVQA4Kqz17ciF5Coh9VMMWJC2Ns5XGWEXo00iaGgSbBlkWakyEI2FiwzJJAKkJfsEcRbwT2H7Nxe5YBvHzNlu1ZojY_brJ9rfrq7ofMEmNOBkLhtbVM8_L8nwsTsWl-Of5ANKuRb8</recordid><startdate>20241105</startdate><enddate>20241105</enddate><creator>BAO XUNDI</creator><creator>KAN XIAOHONG</creator><creator>ZHANG JINGJING</creator><creator>ZHENG YABIAO</creator><scope>EVB</scope></search><sort><creationdate>20241105</creationdate><title>Method for detecting drug resistance of mycobacterium tuberculosis based on nanopore targeted sequencing technology</title><author>BAO XUNDI ; KAN XIAOHONG ; ZHANG JINGJING ; ZHENG YABIAO</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN118895373A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2024</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>COMPOSITIONS THEREOF</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>BAO XUNDI</creatorcontrib><creatorcontrib>KAN XIAOHONG</creatorcontrib><creatorcontrib>ZHANG JINGJING</creatorcontrib><creatorcontrib>ZHENG YABIAO</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>BAO XUNDI</au><au>KAN XIAOHONG</au><au>ZHANG JINGJING</au><au>ZHENG YABIAO</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Method for detecting drug resistance of mycobacterium tuberculosis based on nanopore targeted sequencing technology</title><date>2024-11-05</date><risdate>2024</risdate><abstract>The invention discloses a method for detecting drug resistance of mycobacterium tuberculosis based on a nanopore targeted sequencing technology, and relates to the technical field of microbiological identification. Mycobacterium tuberculosis genome DNA in a sample to be detected is used as a template, and a primer group designed aiming at a drug-resistant gene is used for PCR amplification; the obtained PCR amplification product is subjected to nanopore sequencing platform library building and sequencing, and whether the mycobacterium tuberculosis is resistant to drugs or not is predicted according to sequencing data; according to the invention, a nanopore sequencing platform and a multiplex PCR amplification technology are utilized to realize rapid detection of mycobacterium tuberculosis related drug-resistant genes and mutations, and the detection accuracy and efficiency are improved.
本发明公开了一种基于纳米孔靶向测序技术检测结核分枝杆菌耐药性的方法，涉及微生物鉴定技术领域，本发明以待测样品中的结核分枝杆菌基因组DNA为模板，利用针对耐药基因设计的引物组进行PCR扩增，将得到的PCR扩增产物进行纳米孔测序平台建库和测序，根据测序</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Method for detecting drug resistance of mycobacterium tuberculosis based on nanopore targeted sequencing technology |
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