Method for detecting drug resistance of mycobacterium tuberculosis based on nanopore targeted sequencing technology

The invention discloses a method for detecting drug resistance of mycobacterium tuberculosis based on a nanopore targeted sequencing technology, and relates to the technical field of microbiological identification. Mycobacterium tuberculosis genome DNA in a sample to be detected is used as a template, and a primer group designed aiming at a drug-resistant gene is used for PCR amplification; the obtained PCR amplification product is subjected to nanopore sequencing platform library building and sequencing, and whether the mycobacterium tuberculosis is resistant to drugs or not is predicted according to sequencing data; according to the invention, a nanopore sequencing platform and a multiplex PCR amplification technology are utilized to realize rapid detection of mycobacterium tuberculosis related drug-resistant genes and mutations, and the detection accuracy and efficiency are improved. 本发明公开了一种基于纳米孔靶向测序技术检测结核分枝杆菌耐药性的方法，涉及微生物鉴定技术领域，本发明以待测样品中的结核分枝杆菌基因组DNA为模板，利用针对耐药基因设计的引物组进行PCR扩增，将得到的PCR扩增产物进行纳米孔测序平台建库和测序，根据测序