Primer pair and kit for identifying sex of sea-buckthorn in early stage and application of primer pair and kit

The invention relates to the technical field of gene detection, in particular to a primer pair and a kit for early identification of sex of hippophae rhamnoides and application of the primer pair and the kit. The nucleotide sequences of the primer pair for identifying the sex of the sea buckthorn in...

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Bibliographische Detailangaben
Hauptverfasser: GUO YING, ZHANG XINXIAN, XUE LIANGJIAO, FU FANGFANG, CAO FULIANG, WU YUNHUA, HE JIE, LIU BINGSHUANG, ZHAO ANQI
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to the technical field of gene detection, in particular to a primer pair and a kit for early identification of sex of hippophae rhamnoides and application of the primer pair and the kit. The nucleotide sequences of the primer pair for identifying the sex of the sea buckthorn in the early stage are as shown in SEQ ID NO.1 and SEQ ID NO.2; aiming at sex detection of the sea-buckthorn, the primer pair is successfully constructed, early screening for sex identification of the sea-buckthorn is realized, and the problem of long sex identification period caused by long fruiting period of the sea-buckthorn is solved. 本发明涉及基因检测技术领域,具体涉及一种用于早期鉴别江孜沙棘性别的引物对、试剂盒及应用;本发明用于早期鉴别江孜沙棘性别的引物对的核苷酸序列如SEQ ID NO.1和SEQ ID NO.2所示;本发明针对江孜沙棘性别检测,成功构建了引物对,实现对鉴别江孜沙棘性别的早期筛选,克服了因江孜沙棘结实周期较长导致的性别鉴别的周期长的问题。