Cervical cell gene methylation detection kit and detection method

The invention discloses a cervical cell gene methylation detection kit and a detection method, an MTHFR PCR reaction solution, a PAX1 PCR reaction solution and an SGSH PCR reaction solution provided in the kit contain specific primers and probes for detecting target genes MTHFR, PAX1 and SGSH, the d...

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Bibliographische Detailangaben
Hauptverfasser:	CHEN MENGMENG, LUAN YANSONG, LIU HE, ZHOU YANG, ZHANG BINGQIANG, YU JUNMEI, JIANG XIN, ZHU BAIYANG, MA YONGCHAO, SUN GUOLONG, ZHANG GAOFENG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a cervical cell gene methylation detection kit and a detection method, an MTHFR PCR reaction solution, a PAX1 PCR reaction solution and an SGSH PCR reaction solution provided in the kit contain specific primers and probes for detecting target genes MTHFR, PAX1 and SGSH, the detection sensitivity is high, and the specificity is good. Based on a fluorescent quantitative PCR (Polymerase Chain Reaction) technology, the detection reagent disclosed by the invention is used for carrying out methylation joint detection on three target genes MTHFR, PAX1 and SGSH in a detection sample, so that whether the sample is cancerous or not is judged, differences possibly caused by site selection and a reaction system are effectively supplemented, and the accuracy of cervical cancer screening is improved. 本发明公开了一种宫颈细胞基因甲基化检测试剂盒及检测方法，试剂盒中提供的MTHFR PCR反应液、PAX1 PCR反应液、SGSH PCR反应液中含有检测目标基因MTHFR、PAX1、SGSH的特异性引物和探针，检测灵敏度高，特异性好。基于荧光定量PCR技术，使用本发明检测试剂对检测样本中三个目标基因MTHFR、PAX1、SGSH进行甲基化联合检测，据此判断样本是否癌变，有效补充了可能由于位点选择以及反