Recombinant saccharomyces cerevisiae with beta-glucosidase displayed on surface as well as construction method and application of recombinant saccharomyces cerevisiae

The invention discloses recombinant saccharomyces cerevisiae capable of displaying beta-glucosidase on the surface and a construction method and application of the recombinant saccharomyces cerevisiae, the recombinant saccharomyces cerevisiae adopts a surface display technology, beta-glucosidase BGLY genes from sulfolobus sulphureus are fused with signal peptides, promoters, anchoring proteins and promoter genes, and the recombinant saccharomyces cerevisiae is obtained. And introducing into a modified saccharomyces cerevisiae host cell for surface display expression to obtain the whole-cell catalyst integrating expression, purification and immobilization. The recombinant saccharomyces cerevisiae strain disclosed by the invention can realize efficient immobilization and reuse of beta-glucosidase, also provides a novel whole-cell catalyst for preparing rare ginsenoside Compound K by an enzyme catalysis method, and has a good industrial application prospect. 本发明公开了一种表面展示β-葡萄糖苷酶的重组酿酒酵母及其构建方法和应用，该重组酿酒酵母采用表面展示技术，将来