Method for detecting hesperidin component of modified Huoxiang Zhengqi pill based on HPLC (High Performance Liquid Chromatography) method

The invention relates to the technical field of detection of hesperidin components of modified Huoxiang Zhengqi pills, in particular to a method for detecting the hesperidin components of the modified Huoxiang Zhengqi pills based on an HPLC (High Performance Liquid Chromatography) method, which comprises the following steps: preparing a reference solution and a test solution, and feeding the prepared reference solution and test solution under preset chromatographic conditions, the method is characterized in that according to the chromatographic conditions, a WAT054275 column with octadecylsilane chemically bonded silica as a filler is adopted, the column temperature is controlled at 25-35 DEG C, the flow rate is 1.0 ml/min, the detection wavelength is 225-285 nm, the sample size is 3-15 [mu] L, a microporous filter membrane with the size of 0.4-0.5 [mu] m is used for filtration during sample introduction, acetonitrile is used as a mobile phase A, the column temperature is controlled at 25-35 DEG C, the flow r