Preparation method of pseudoviruses of human herpes viruses 6, 7 and 8 and application of pseudoviruses in molecular detection
The invention provides human herpes virus 6, 7 and 8 pseudoviruses as well as a preparation method and application thereof, and relates to the technical field of pathogen nucleic acid detection.The HHV6/HHV7/HHV8 pseudoviruses provided by the invention contain independent conserved gene amplificatio...
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| creator | DONG YINGYING LI HUITING WANG XINLE SUN YUNXIA FU XINYUE LI KAITONG ZHANG RUIRUI LIU MINGYUE ZHANG TONG WANG YANHUI PANG LIN CUI MENGSHAN RAO CHUNMING CHEN XIAOFEI CAO YIDAN ZHOU JIN |
| description | The invention provides human herpes virus 6, 7 and 8 pseudoviruses as well as a preparation method and application thereof, and relates to the technical field of pathogen nucleic acid detection.The HHV6/HHV7/HHV8 pseudoviruses provided by the invention contain independent conserved gene amplification sequences of the HHV6 virus, the HHV7 virus and the HHV8 virus, and can be applied to detection of nucleic acid sequences of three common human herpes viruses HHV6/HHV7/HHV8 viruses in a sample. And using as a positive control. Meanwhile, the practical application of the HHV6/HHV7/HHV8 pseudovirus in qPCR (quantitative polymerase chain reaction) detection can be used for quantitative analysis of the three human herpes viruses in experimental animal models, is also suitable for human-derived virus factor inspection of animal cell matrixes for biological product production and animal cells for verification, and plays an important role in safety of biological products.
本发明提供了一种人疱疹病毒6型、7型和8型假病毒、制备方法及其应用，涉及病原体核酸检测技术领域 |
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本发明提供了一种人疱疹病毒6型、7型和8型假病毒、制备方法及其应用，涉及病原体核酸检测技术领域</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2024</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20241025&DB=EPODOC&CC=CN&NR=118834843A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76289</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20241025&DB=EPODOC&CC=CN&NR=118834843A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>DONG YINGYING</creatorcontrib><creatorcontrib>LI HUITING</creatorcontrib><creatorcontrib>WANG XINLE</creatorcontrib><creatorcontrib>SUN YUNXIA</creatorcontrib><creatorcontrib>FU XINYUE</creatorcontrib><creatorcontrib>LI KAITONG</creatorcontrib><creatorcontrib>ZHANG RUIRUI</creatorcontrib><creatorcontrib>LIU MINGYUE</creatorcontrib><creatorcontrib>ZHANG TONG</creatorcontrib><creatorcontrib>WANG YANHUI</creatorcontrib><creatorcontrib>PANG LIN</creatorcontrib><creatorcontrib>CUI MENGSHAN</creatorcontrib><creatorcontrib>RAO CHUNMING</creatorcontrib><creatorcontrib>CHEN XIAOFEI</creatorcontrib><creatorcontrib>CAO YIDAN</creatorcontrib><creatorcontrib>ZHOU JIN</creatorcontrib><title>Preparation method of pseudoviruses of human herpes viruses 6, 7 and 8 and application of pseudoviruses in molecular detection</title><description>The invention provides human herpes virus 6, 7 and 8 pseudoviruses as well as a preparation method and application thereof, and relates to the technical field of pathogen nucleic acid detection.The HHV6/HHV7/HHV8 pseudoviruses provided by the invention contain independent conserved gene amplification sequences of the HHV6 virus, the HHV7 virus and the HHV8 virus, and can be applied to detection of nucleic acid sequences of three common human herpes viruses HHV6/HHV7/HHV8 viruses in a sample. And using as a positive control. Meanwhile, the practical application of the HHV6/HHV7/HHV8 pseudovirus in qPCR (quantitative polymerase chain reaction) detection can be used for quantitative analysis of the three human herpes viruses in experimental animal models, is also suitable for human-derived virus factor inspection of animal cell matrixes for biological product production and animal cells for verification, and plays an important role in safety of biological products.
本发明提供了一种人疱疹病毒6型、7型和8型假病毒、制备方法及其应用，涉及病原体核酸检测技术领域</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>COMPOSITIONS THEREOF</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2024</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjDsLwjAUhbM4iPofrrsOpUWzSlGcxMG9XJJbEkiTSx6O_nZbH5OLyzmcj8M3F49rJMaI2QYPA2UTNIQeOFHR4W5jSZQmYMqAHgxFHveX7zawB_Qa5CuR2Vn1Vv047KgPjlRxGEFTJjX9lmLWo0u0-vRCrE_HW3veEoeOEqMiT7lrL1UlZd3Ipj7U_3yeb0RIUg</recordid><startdate>20241025</startdate><enddate>20241025</enddate><creator>DONG YINGYING</creator><creator>LI HUITING</creator><creator>WANG XINLE</creator><creator>SUN YUNXIA</creator><creator>FU XINYUE</creator><creator>LI KAITONG</creator><creator>ZHANG RUIRUI</creator><creator>LIU MINGYUE</creator><creator>ZHANG TONG</creator><creator>WANG YANHUI</creator><creator>PANG LIN</creator><creator>CUI MENGSHAN</creator><creator>RAO CHUNMING</creator><creator>CHEN XIAOFEI</creator><creator>CAO YIDAN</creator><creator>ZHOU JIN</creator><scope>EVB</scope></search><sort><creationdate>20241025</creationdate><title>Preparation method of pseudoviruses of human herpes viruses 6, 7 and 8 and application of pseudoviruses in molecular detection</title><author>DONG YINGYING ; 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And using as a positive control. Meanwhile, the practical application of the HHV6/HHV7/HHV8 pseudovirus in qPCR (quantitative polymerase chain reaction) detection can be used for quantitative analysis of the three human herpes viruses in experimental animal models, is also suitable for human-derived virus factor inspection of animal cell matrixes for biological product production and animal cells for verification, and plays an important role in safety of biological products.
本发明提供了一种人疱疹病毒6型、7型和8型假病毒、制备方法及其应用，涉及病原体核酸检测技术领域</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Preparation method of pseudoviruses of human herpes viruses 6, 7 and 8 and application of pseudoviruses in molecular detection |
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