Preparation method of pseudoviruses of human herpes viruses 6, 7 and 8 and application of pseudoviruses in molecular detection

The invention provides human herpes virus 6, 7 and 8 pseudoviruses as well as a preparation method and application thereof, and relates to the technical field of pathogen nucleic acid detection.The HHV6/HHV7/HHV8 pseudoviruses provided by the invention contain independent conserved gene amplification sequences of the HHV6 virus, the HHV7 virus and the HHV8 virus, and can be applied to detection of nucleic acid sequences of three common human herpes viruses HHV6/HHV7/HHV8 viruses in a sample. And using as a positive control. Meanwhile, the practical application of the HHV6/HHV7/HHV8 pseudovirus in qPCR (quantitative polymerase chain reaction) detection can be used for quantitative analysis of the three human herpes viruses in experimental animal models, is also suitable for human-derived virus factor inspection of animal cell matrixes for biological product production and animal cells for verification, and plays an important role in safety of biological products. 本发明提供了一种人疱疹病毒6型、7型和8型假病毒、制备方法及其应用，涉及病原体核酸检测技术领域