PCV1-PCV3-TTSuV1-TTSuV2 polygene pseudovirus as well as preparation method and application thereof
The invention provides a PCV1-PCV3-TTSuV1-TTSuV2 polygene pseudovirus as well as a preparation method and application thereof, and relates to the technical field of biology, and the polygene pseudovirus provided by the invention can be applied to detection of various DNA viruses such as a porcine ci...
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| creator | LI YAOLING WANG YAO FANG JIQING RAO CHUNMING SUN YUNXIA WEI XIAJIE LI KAITONG WANG NING YANG YING ZHOU JIN YUAN ZIWEI |
| description | The invention provides a PCV1-PCV3-TTSuV1-TTSuV2 polygene pseudovirus as well as a preparation method and application thereof, and relates to the technical field of biology, and the polygene pseudovirus provided by the invention can be applied to detection of various DNA viruses such as a porcine circovirus type I, a porcine circovirus type III, a porcine torque teno virus type I and a porcine torque teno virus type II. The invention has the following advantages: (1) the replication-deficient adenovirus can be replicated in a specific packaging cell, so the preparation process is simple and economical; (2) the target nucleic acid fragment is subjected to sequence optimization and does not express protein, so that the recombinant replication-deficient adenovirus has no pathogenic risk; and (3) the prepared positive control pseudovirus contains a plurality of nucleic acid fragments, and can be applied to a plurality of molecular detection tests through one-time preparation. The invention provides an exogenous v |
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The invention has the following advantages: (1) the replication-deficient adenovirus can be replicated in a specific packaging cell, so the preparation process is simple and economical; (2) the target nucleic acid fragment is subjected to sequence optimization and does not express protein, so that the recombinant replication-deficient adenovirus has no pathogenic risk; and (3) the prepared positive control pseudovirus contains a plurality of nucleic acid fragments, and can be applied to a plurality of molecular detection tests through one-time preparation. 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The invention has the following advantages: (1) the replication-deficient adenovirus can be replicated in a specific packaging cell, so the preparation process is simple and economical; (2) the target nucleic acid fragment is subjected to sequence optimization and does not express protein, so that the recombinant replication-deficient adenovirus has no pathogenic risk; and (3) the prepared positive control pseudovirus contains a plurality of nucleic acid fragments, and can be applied to a plurality of molecular detection tests through one-time preparation. 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The invention has the following advantages: (1) the replication-deficient adenovirus can be replicated in a specific packaging cell, so the preparation process is simple and economical; (2) the target nucleic acid fragment is subjected to sequence optimization and does not express protein, so that the recombinant replication-deficient adenovirus has no pathogenic risk; and (3) the prepared positive control pseudovirus contains a plurality of nucleic acid fragments, and can be applied to a plurality of molecular detection tests through one-time preparation. The invention provides an exogenous v</abstract><oa>free_for_read</oa></addata></record> |
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| language | chi ; eng |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | PCV1-PCV3-TTSuV1-TTSuV2 polygene pseudovirus as well as preparation method and application thereof |
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