PCV1-PCV3-TTSuV1-TTSuV2 polygene pseudovirus as well as preparation method and application thereof

The invention provides a PCV1-PCV3-TTSuV1-TTSuV2 polygene pseudovirus as well as a preparation method and application thereof, and relates to the technical field of biology, and the polygene pseudovirus provided by the invention can be applied to detection of various DNA viruses such as a porcine circovirus type I, a porcine circovirus type III, a porcine torque teno virus type I and a porcine torque teno virus type II. The invention has the following advantages: (1) the replication-deficient adenovirus can be replicated in a specific packaging cell, so the preparation process is simple and economical; (2) the target nucleic acid fragment is subjected to sequence optimization and does not express protein, so that the recombinant replication-deficient adenovirus has no pathogenic risk; and (3) the prepared positive control pseudovirus contains a plurality of nucleic acid fragments, and can be applied to a plurality of molecular detection tests through one-time preparation. The invention provides an exogenous v