Cryopreservation device and cryopreservation method for programmed cooling of stem cells

The invention discloses a cryopreservation device and a cryopreservation method for programmed cooling of stem cells, the device comprises a box body with an opening in the front side surface, an opening and closing door is mounted at the opening of the box body, and a compressor cooling area, an isolation area, a sample placement area and a liquid nitrogen cooling area are sequentially arranged in the box body from top to bottom; the isolation area comprises a supporting frame, a plurality of heat insulation plates with adjustable gaps are rotationally connected into the supporting frame, and the heat insulation plates rotate to set angles, so that communication and isolation between the compressor cooling area and the sample placement area can be realized. According to the device, cryopreserved samples can be directly placed in the device at normal temperature for programmed cooling, toxicity interference of isopropanol in the cooling box to experimenters is avoided, liquid nitrogen does not need to be tran