Primer probe combination for real-time fluorescence RAA (Reverse Amplification Amplification) detection of circle virus human type-1 and application of primer probe combination

The invention discloses a primer probe combination for real-time fluorescence RAA detection of a circle virus human type-1 and application of the primer probe combination, and relates to the technical field of biological detection. The primer probe combination comprises an upstream primer F4, a downstream primer R1 and a probe P, the nucleotide sequence of the upstream primer F4 is as shown in SEQ ID NO.7; the nucleotide sequence of the downstream primer R1 is as shown in SEQ ID NO. 8; and the nucleotide sequence of the probe P is as shown in SEQ ID NO. 3. The primer probe combination can realize real-time fluorescence RAA detection of the circle virus human source type 1, and has the advantages of rapid operation, high sensitivity and strong specificity. The invention provides a new technical means for accurately and rapidly detecting GyH1. 本发明公开了一种用于圆圈病毒人源1型实时荧光RAA检测的引物探针组合及其应用，涉及生物检测技术领域。该引物探针组合包括上游引物F4、下游引物R1和探针P；所述上游引物F4的核苷酸序列如SEQ ID NO.7所示；所述下游引物R1的核苷酸序列如SEQ ID NO.8所示；所述探针P的核苷酸序列如SEQ ID NO.3所示。利用该引物探针组合