Method for separating and purifying sea buckthorn flavone from sea buckthorn pomace
The invention discloses a method for separating and purifying sea buckthorn flavone from sea buckthorn pomace, and belongs to the technical field of plant extraction. Comprising the following steps: degreasing hippophae rhamnoides fruit residues with petroleum ether, recovering a solvent, and drying...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention discloses a method for separating and purifying sea buckthorn flavone from sea buckthorn pomace, and belongs to the technical field of plant extraction. Comprising the following steps: degreasing hippophae rhamnoides fruit residues with petroleum ether, recovering a solvent, and drying; adding a biological enzyme and a buffer solution, carrying out an enzymatic hydrolysis reaction, and filtering to obtain an enzymatic hydrolysate; adding ethanol and metal salt into the enzymatic hydrolysate, adjusting the pH value, stirring for complex reaction, centrifuging, and collecting precipitate to obtain a primary crude extract; adding the primary crude extract into a mixed solution of ethylene glycol, succinic acid and 1-methylimidazole, carrying out ultrasonic extraction, centrifuging, concentrating and drying to obtain a secondary crude extract; and dissolving the secondary crude extract in an ethanol solution, adding EDTA (Ethylene Diamine Tetraacetic Acid), carrying out decomplexing reaction under t |
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