Method for creating corn germplasm with high starch freeze-thaw stability and related biological material thereof
The invention discloses a method for creating a corn germplasm with high starch freeze-thaw stability and a related biological material of the corn germplasm. Specifically, the invention discloses application of proteins with amino acid sequences of SEQ ID No.2 and SEQ ID No.5 in regulation and cont...
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| creator | XIE CHUANXIAO MA MINGZHENG XU XIAOJIE LIU CHANGLIN ZHU JINJIE QI XIANTAO HUANG JING |
| description | The invention discloses a method for creating a corn germplasm with high starch freeze-thaw stability and a related biological material of the corn germplasm. Specifically, the invention discloses application of proteins with amino acid sequences of SEQ ID No.2 and SEQ ID No.5 in regulation and control of the freeze-thaw stability of corn. According to the invention, the Zmsu2 gene and the Zmwx gene are knocked out by using a CRISPR/Cas9 gene editing technology to obtain the corn mutant, and the result shows that the Zmsu2 gene and the Zmwx gene are knocked out to change the straight chain proportion and structure of starch, so that the freeze-thaw stability of corn can be remarkably improved. According to the method, the corn germplasm with high starch freeze-thaw stability is successfully created, material accumulation is provided for corn breeding, an accurate, efficient and safe technical method is provided for corn freeze-thaw stability breeding or germplasm resource improvement of freeze-thaw stable typ |
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Specifically, the invention discloses application of proteins with amino acid sequences of SEQ ID No.2 and SEQ ID No.5 in regulation and control of the freeze-thaw stability of corn. According to the invention, the Zmsu2 gene and the Zmwx gene are knocked out by using a CRISPR/Cas9 gene editing technology to obtain the corn mutant, and the result shows that the Zmsu2 gene and the Zmwx gene are knocked out to change the straight chain proportion and structure of starch, so that the freeze-thaw stability of corn can be remarkably improved. 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Specifically, the invention discloses application of proteins with amino acid sequences of SEQ ID No.2 and SEQ ID No.5 in regulation and control of the freeze-thaw stability of corn. According to the invention, the Zmsu2 gene and the Zmwx gene are knocked out by using a CRISPR/Cas9 gene editing technology to obtain the corn mutant, and the result shows that the Zmsu2 gene and the Zmwx gene are knocked out to change the straight chain proportion and structure of starch, so that the freeze-thaw stability of corn can be remarkably improved. According to the method, the corn germplasm with high starch freeze-thaw stability is successfully created, material accumulation is provided for corn breeding, an accurate, efficient and safe technical method is provided for corn freeze-thaw stability breeding or germplasm resource improvement of freeze-thaw stable typ</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE |
| title | Method for creating corn germplasm with high starch freeze-thaw stability and related biological material thereof |
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