Method for creating corn germplasm with high starch freeze-thaw stability and related biological material thereof

The invention discloses a method for creating a corn germplasm with high starch freeze-thaw stability and a related biological material of the corn germplasm. Specifically, the invention discloses application of proteins with amino acid sequences of SEQ ID No.2 and SEQ ID No.5 in regulation and control of the freeze-thaw stability of corn. According to the invention, the Zmsu2 gene and the Zmwx gene are knocked out by using a CRISPR/Cas9 gene editing technology to obtain the corn mutant, and the result shows that the Zmsu2 gene and the Zmwx gene are knocked out to change the straight chain proportion and structure of starch, so that the freeze-thaw stability of corn can be remarkably improved. According to the method, the corn germplasm with high starch freeze-thaw stability is successfully created, material accumulation is provided for corn breeding, an accurate, efficient and safe technical method is provided for corn freeze-thaw stability breeding or germplasm resource improvement of freeze-thaw stable typ