Purification method of horse anti-snake venom immune globulin preparation

The invention provides a purification method of horse anti-snake venom immune globulin. The purification method is characterized by comprising the following steps: A, pretreatment of horse immune plasma; b, gastric enzyme digestion; c, heating for denaturation; d, filtrate treatment; e, hydrophobic chromatography; f, ultrafiltration; g, preparing a stock solution; and H, preparing and sub-packaging a semi-finished product. The adopted hydrophobic chromatography technology belongs to adsorption chromatography, and compared with affinity chromatography and ion exchange chromatography, in the separation process of hydrophobic chromatography, the interaction force between a separation medium and protein molecules is weak, the protein is not prone to violent change of molecular structures in chromatographic separation, and the protein is not prone to losing activity; the prepared horse anti-snake venom immune globulin preparation has the characteristics of high purity, high specific activity and better product qua