Cell aging test kit and detection method thereof

The invention discloses a cell senescence test kit and a detection method thereof, particularly relates to the technical field of cell function detection, and aims to solve the problems that in the prior art, a kit is incompatible with polystyrene containers such as perforated plates and pipettes. T...

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Bibliographische Detailangaben
Hauptverfasser: SUN JIAOJIAO, ZHANG FENG, SUN YIJUN, ZHANG CHUNJI
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a cell senescence test kit and a detection method thereof, particularly relates to the technical field of cell function detection, and aims to solve the problems that in the prior art, a kit is incompatible with polystyrene containers such as perforated plates and pipettes. The kit comprises a beta-galactosidase fixing solution, a beta-galactosidase staining solution and a 5-bromine-4-chloro-3-indolyl-beta-D-galactopyranoside solution, wherein the beta-galactosidase fixing solution, the beta-galactosidase staining solution and the 5-bromine-4-chloro-3-indolyl-beta- The method is simple and convenient, does not separate out crystals, is deep in positive cell dyeing degree, can be compatible with common containers made of polystyrene materials, such as perforated plates and pipettes for cell culture, and can effectively avoid the conditions of weak dyeing, unstable dyeing effect and the like caused by incompatibility with the perforated plates, the pipettes and the like. 本发明公开了一种细胞衰老测试试剂