QPCR (quantitative polymerase chain reaction) detection method for Sirt1 mRNA (messenger ribonucleic acid) expression for cynomolgus monkey semen quality evaluation

The invention provides a qPCR (quantitative polymerase chain reaction) detection method for Sirt1 mRNA (messenger ribonucleic acid) expression for macaca fascicularis semen quality evaluation, which belongs to a detection method, and specifically comprises the following steps of: performing real-time fluorescent quantitative PCR amplification by using a PCR primer combination by taking cDNA (complementary deoxyribonucleic acid) obtained by reverse transcription synthesis of total RNA (ribonucleic acid) extracted from fresh macaca fascicularis semen as a template, ct values, dissolution peaks and amplification efficiency of SIRT1 gene segments and reference gene GAPDH segments are obtained, and a homogenized delta delta C (t) value expressed by SIRT1 gene multiples is obtained through conventional treatment, so that a SIRT1 gene transcriptional level relative expression value is obtained. And an effective way is provided for researching the function of the cynomolgus monkey SIRT1 and the influence of related m