Application of multi-gene typing detection composition

The invention provides application of a multi-genotyping detection composition, and belongs to the technical field of gene detection.The multi-genotyping detection composition is applied to a PCR reaction system and comprises a universal quenching probe, a plurality of specific amplification primers and a plurality of universal signal probes, the universal quenching probe and the universal signal probe are oligonucleotide chains which are not paired with gene sequences of any known species to generate amplification reaction; the 5'end of the general signal probe is respectively marked with different fluorescence signal groups, and the fluorescence signal of each fluorescence signal group can be quenched by the quenching group of the quenching probe. According to the invention, a universal probe is used for simultaneously carrying out typing detection on different genotypes in the same reaction tube, so that the detection efficiency is improved, the reagent cost is reduced, and the detection range is expanded.