High-infiltration-enhancing recombinant protein liposome and preparation method thereof

The invention discloses a high-infiltration-enhancing recombinant protein liposome and a preparation method thereof. The high-penetration-enhancing recombinant protein liposome is prepared from the following components in percentage by weight: 6%-10% of phosphatidylcholine, 25%-30% of glycerol, 3.2%-3.5% of recombinant protein, 7%-8% of 1, 2-pentanediol, 0.8%-1.0% of sodium hyaluronate and water. The phosphatidylcholine is used for spontaneously forming the polycystic liposome in water, the recombinant protein is wrapped in an inner cavity, then the liposome with the uniform particle size and the nanoscale particle size is obtained through high-pressure homogenization, meanwhile, under the wrapping effect of the sodium hyaluronate, the long-term stability of the liposome is improved, and permeation of the recombinant protein in skin is promoted. 本发明公开了一种高促渗的重组蛋白脂质体及其制备方法。所述的高促渗的重组蛋白脂质体由磷脂酰胆碱6%~10%，甘油25%~30%，重组蛋白3.2%~3.5%，1,2-戊二醇7~8%，透明质酸钠0.8~1.0%和水组成。本发明通过使用磷脂酰胆碱在水中自发形成多囊室脂质体，将重组蛋白包裹在内腔，再经高压均质得到粒径均一且为纳米级别粒径的脂