Reagent for detecting turbot thrombus cells and application thereof

The invention provides a reagent for detecting turbot thrombus and application of the reagent. The invention discloses a marker Mpl which comes from turbot and is suitable for detecting turbot thrombus or thrombus cells. Based on the Mpl protein, the inventor obtains an Mpl-out fragment as an immunogen, and efficient recombinant expression can be realized by recombinant expression of the fragment. Meanwhile, a polyclonal antibody with high titer can be obtained by taking the fragment as an immunogen, and the polyclonal antibody has high titer and excellent binding performance. 本发明提供了一种检测大菱鲆血栓的试剂及其应用。本发明揭示了来自大菱鲆的适于检测大菱鲆血栓或血栓细胞的标志物Mpl。基于该Mpl蛋白，发明人获得了Mpl-out片段作为免疫原，重组表达该片段能够实现高效的重组表达。同时，以该片段作为免疫原可获得高效价的多克隆抗体，其具有高的效价且结合性能优异。