Primer pair for identifying tomato plexiviridae virus and application of primer pair

The invention discloses a primer pair for identifying a tomato psiriviridae virus and application of the primer pair. The invention provides a primer pair I or a primer pair II. The primer pair I is composed of a primer as shown in SEQ ID NO: 1 and a primer as shown in SEQ ID NO: 2; the primer pair...

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Bibliographische Detailangaben
Hauptverfasser:	FANG ZHIPENG, CAI HONGJIAO, YE MINGHUI, HUANG PENGYING, LIAO FURONG, SHEN JIANGUO
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a primer pair for identifying a tomato psiriviridae virus and application of the primer pair. The invention provides a primer pair I or a primer pair II. The primer pair I is composed of a primer as shown in SEQ ID NO: 1 and a primer as shown in SEQ ID NO: 2; the primer pair II is composed of a primer as shown in SEQ ID NO: 3 and a primer as shown in SEQ ID NO: 4. The primer pair provided by the invention can realize RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection on the tomato plexiviridae virus, is combined with sequence determination to identify virus types, and has the advantages of good broad spectrum, high sensitivity and strong specificity. 本发明公开了用于鉴定番茄丛矮病毒科病毒的引物对及其应用。本发明提供了引物对Ⅰ或引物对Ⅱ。引物对Ⅰ由SEQ ID NO：1所示的引物和SEQ ID NO：2所示的引物组成；引物对Ⅱ由SEQ ID NO：3所示的引物和SEQ ID NO：4所示的引物组成。本发明提供的引物对可对番茄丛矮病毒科病毒实现RT-PCR检测，结合序列测定鉴定病毒种类，具有广谱性好、灵敏度高、特异性强的优点。