Construction method of acanthopanax genetic transformation system

The invention discloses a construction method of an acanthopanax genetic transformation system, and belongs to the field of plant genetic transformation. According to the method, stems and leaves of the acanthopanax senticosus are dip-dyed by agrobacterium under a vacuum or ultrasonic condition and then are transferred into a co-culture medium for co-culture, bacteria removal is performed, and resistant calluses are screened; wherein in the dip dyeing and co-culture stages, lanthanum chloride is added into an agrobacterium dip dyeing solution and a co-culture medium; and continuously culturing the screened resistant calluses to obtain the resistant acanthopanax senticosus seedlings. According to the method, the acanthopanax genetic transformation system is successfully constructed, the resistance acanthopanax seedlings with stable inheritance are obtained, the adventitious roots of the resistance acanthopanax seedlings are large in quantity and good in growth vigor, and the method has important significance o