Preparation and identification method of rice blast resistant rice

The invention provides a preparation method of rice blast resistant rice. By inhibiting the expression of the DHD6 gene in rice or constructing a defective plant of the DHD6 gene, a rice plant with rice blast resistance can be obtained. The CDS sequence of the DHD6 gene is as shown in SEQ ID NO: 1, or the sequence of the protein coded by the DHD6 gene is as shown in SEQ ID NO: 2. Rice plants with improved rice blast resistance can be identified by detecting the function deficiency of the DHD6 gene. The invention also provides a mutated DHD6 gene, and the CDS sequence of the mutated DHD6 gene is as shown in SEQ ID NO: 3, or the sequence of the protein coded by the mutated DHD6 gene is as shown in SEQ ID NO: 4. A wild type DHD6 gene is replaced by the mutated gene through a genetic engineering means, and a rice plant with disease resistance can be constructed. 本发明提供了一种抗稻瘟病水稻的制备方法。通过在水稻中抑制DHD6基因表达或者构建DHD6基因的缺陷型植株，可以获得具有抗稻瘟病性质的水稻植株。所述DHD6基因的CDS序列如SEQ ID NO：1所示，或者所述DHD6基因所编码的蛋白质的序列如SEQ ID NO：2所示。通过检测DHD6基因功能缺失可以鉴定