Preparation method and application of Fe3O4 (at) Phe (at) lipase interface floating microspheres

The invention belongs to the technical field of enzyme immobilization, and particularly discloses a preparation method and application of Fe3O4 (at) Phe (at) lipase interface floating microspheres, and the preparation method comprises the following steps: preparing hydrophobic nano magnetic particle...

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Bibliographische Detailangaben
Hauptverfasser:	HUANG XIAOHUI, HAKUMATSU HISASHI, CHI CHANGBIAO, YU HAOZHOU, HUO BUQING, ZHOU XUANHONG, CHEN GUO, HUANG ZIYIN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention belongs to the technical field of enzyme immobilization, and particularly discloses a preparation method and application of Fe3O4 (at) Phe (at) lipase interface floating microspheres, and the preparation method comprises the following steps: preparing hydrophobic nano magnetic particles Fe3O4 (at) Phe; preparing a biological compound Fe3O4 (at) Phe (at) lipase; and carrying out 3D printing on the Fe3O4 (at) Phe (at) lipase interface floating microspheres. The Fe3O4 (at) Phe (at) lipase interface floating microspheres prepared by the invention can enable degradation reactions of all lipases to be carried out on an oil-water interface, and the interface reaction is accelerated, so that the degradation efficiency of the lipases is improved; the Fe3O4 (at) Phe (at) lipase interface floating microspheres prepared by the method can be magnetically separated through an external magnetic field, and can be quickly recycled and reused, so that the industrial application cost of degrading grease by lipase