Separation, in-vitro culture and identification of adipose-derived mesenchymal stem cells from sheep early embryo

The invention discloses separation, in-vitro culture and identification of adipose-derived mesenchymal stem cells from a sheep early embryo, and relates to the technical field of stem cell culture. The adipose-derived stem cells are separated by a method of separating adipose tissue of the tail (hip...

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Hauptverfasser:	LI YINGYING, HAN JILONG, ZHANG JIE, DOU MINGQIANG, YANG MIN, HUR HYUCK, YANG SURONG, LI TIANTIAN, DONG HUAJIAO, RONG XURAN, LIANG QIANQIAN, WANG XIANSHU, YANG HAICHEN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses separation, in-vitro culture and identification of adipose-derived mesenchymal stem cells from a sheep early embryo, and relates to the technical field of stem cell culture. The adipose-derived stem cells are separated by a method of separating adipose tissue of the tail (hip) part of fat tail (hip) type sheep with the gestational age of 70-75 days and combining pancreatin digestion and tissue adherence, and are cultured by using a cell culture solution with simple components, so that a simple, convenient and efficient adipose-derived stem cell culture method is provided for beginners. The method has the characteristics of being wide in material source, simple and rapid to operate, high in repeatability, good in activity of separated cells and strong in dryness, subculture can be carried out within 6 days, the purpose of rapidly obtaining a large number of adipose-derived mesenchymal stem cells in a short time is achieved, and the cultured adipose-derived mesenchymal stem cells can be