Preparation method based on alpaca IgG antibody

The invention belongs to the technical field of immunology, and particularly relates to a preparation method of an alpaca IgG antibody. The alpaca-based IgG antibody comprises two kinds of antibodies, namely MGNb and RGNb. During preparation, modified affinity purification histidine tags 6xHE are inserted into the C ends of gene editing sequences of the two nano antibodies, so that subsequent recombinant expression is facilitated. According to the invention, an escherichia coli prokaryotic expression system is utilized, the inventor performs heterologous expression preparation on alpaca-derived IgG, and the prepared IgG antibody and the existing commercial IgG antibody are compared and evaluated. Results show that the prokaryotic expression system can be well utilized for heterologous expression to obtain the antibody, enzyme labeling can be effectively carried out, and the activity of the prepared antibody is equivalent to that of an existing commercial IgG antibody. The results show that related antibody pr