Low-temperature preserving fluid and preserving method for dog sperms

The invention relates to a dog sperm low-temperature preservation solution and a preservation method. The dog sperm low-temperature preservation solution is an aqueous solution containing the following components in concentration: 0.25 mg/ml-0. 50 mg/ml of astragalus polysaccharide, 18 v/v%-22 v/v%...

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Bibliographische Detailangaben
Hauptverfasser: HU MINHUA, WANG RONGGEN, ZHOU ZHIDONG, ZHAO ZHIHONG, LIU RUIYAN, WU YANFANG, NI QINGCHUN, HUANG XIAOGANG
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention relates to a dog sperm low-temperature preservation solution and a preservation method. The dog sperm low-temperature preservation solution is an aqueous solution containing the following components in concentration: 0.25 mg/ml-0. 50 mg/ml of astragalus polysaccharide, 18 v/v%-22 v/v% of yolk, 28 mg/ml-32 mg/ml of trihydroxymethyl aminomethane, 15 mg/ml-20 mg/ml of citric acid and 10 mg/ml-15 mg/ml of fructose. The preservation method comprises the following steps: putting the dog sperms into the low-temperature preservation solution, and preserving at the temperature of 2-8 DEG C. The dog sperm low-temperature preservation solution obtained by adding a proper amount of astragalus polysaccharide (0.25 mg/ml-0. 50 mg/ml) into a diluent can form an environment for effectively protecting dog sperms in a low-temperature state, a better low-temperature preservation effect which is not achieved by a conventional antioxidant additive is obtained, and after the dog sperms are preserved for 8 days under