Method for determining dissolution rate of bergenin in compound bergenin tablet

The invention discloses a method for determining the dissolution rate of bergenin in a compound bergenin tablet, which adopts HPLC (High Performance Liquid Chromatography) detection and specifically comprises the following steps: 1) preparing a reference solution; 2) preparing a test solution; (3) r...

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Hauptverfasser: YANG MEI, CAO JUNHAN, ZHAO XINQING, LI XIN, LI PING, ZOU WEI, XIAO YAO, LIU XIUHAN, SU LI, XIE LI
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a method for determining the dissolution rate of bergenin in a compound bergenin tablet, which adopts HPLC (High Performance Liquid Chromatography) detection and specifically comprises the following steps: 1) preparing a reference solution; 2) preparing a test solution; (3) respectively sucking the reference solution and the test solution and injecting into a high performance liquid chromatograph; chromatographic conditions are as follows: a chromatographic column takes octadecylsilane chemically bonded silica as a filler; and a mobile phase is 0.5% triethylamine solution-methanol with the volume ratio of 82: 18. According to the method, bergenin is effectively separated in a chromatogram through a specific sample solution preparation method and chromatographic conditions, and interference of other components is avoided, so that the dissolution rate of bergenin in the compound bergenin tablet is accurately measured, the sensitivity is high, a basis is provided for quality control of th