Method for evaluating muscle fiber characteristics of pseudosciaena crocea

The invention relates to a method for evaluating muscle fiber characteristics of pseudosciaena crocea. The method comprises the following steps: 1) collecting a sample; (2) dehydrating and waxing; (3) paraffin embedding; 4) paraffin slicing; 5) dewaxing and washing; 6) dyeing; (7) dehydrating and se...

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Bibliographische Detailangaben
Hauptverfasser: CHEN SHUN, YAN XIAOJUN, ZHU QINGLING, JIANG LIHUA, ZHENG JIALANG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to a method for evaluating muscle fiber characteristics of pseudosciaena crocea. The method comprises the following steps: 1) collecting a sample; (2) dehydrating and waxing; (3) paraffin embedding; 4) paraffin slicing; 5) dewaxing and washing; 6) dyeing; (7) dehydrating and sealing; 8) image acquisition; and (9) the indexes are calculated through Photoshop. The ratio of the muscle fiber area to the muscle fiber void area is introduced as an evaluation index, and compared with traditional texture feature detection, electron microscope analysis, body composition analysis and dyeing observation of the density and diameter of the muscle fibers, the evaluation method which is high in cost benefit, easy and convenient to operate, high in repeatability and sensitive is provided. According to the method, the evaluation process is accelerated, the cost is reduced, the accuracy and reliability of the result are improved, and the market quality of the pseudosciaena crocea can be distinguished and