Bovine nodular skin disease virus loop-mediated isothermal amplification primer group and application thereof

The invention provides a bovine nodular skin disease virus loop-mediated isothermal amplification primer group and application thereof, and relates to the technical field of biological detection.The bovine nodular skin disease virus loop-mediated isothermal amplification primer group comprises an ou...

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Hauptverfasser: JIANG DINGFENG, ZHENG JINLAI, LIANG SHENG, WU GUOZHANG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention provides a bovine nodular skin disease virus loop-mediated isothermal amplification primer group and application thereof, and relates to the technical field of biological detection.The bovine nodular skin disease virus loop-mediated isothermal amplification primer group comprises an outer primer F3, an outer primer B3, an inner primer FIP, an inner primer BIP, a loop primer LF and a loop primer LB. The primer group is used for detecting an LSDV101 type conserved sequence; the bovine nodular skin disease virus can be distinguished from the O-type foot and mouth disease virus, the peste des petits ruminants virus and the goat pox virus, and good specificity is achieved; the sensitivity reaches 1.0 * 100 copies/mu L (i.e., a single copy template), is more sensitive than a colloidal gold test strip, breaks through the dependence on equipment, and is more accurate in detection result. The technical problem that the detection method in the prior art is difficult to distinguish the bovine nodular skin